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Phis2-p53

Webb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 … Webb2、 酵母双杂交 酵母双杂交及系统是一种鉴定和检测蛋白质相互作用的研究方法，因其具有灵敏性高、功能强大、适用范围广等特点，现已被应用于多个研究领域。 ①核蛋白酵母 …

Y187-pHis2YeastOne-HybridMediaKit 使用说明书

Webb5 apr. 2024 · The Y187 strain co-expressing pGADT7-LkARF7 and pHIS2-LkBBM1 and the strain co-expressing pGADT7-LkARF19 and pHIS2-LkBBM1 grew normally on TDO medium containing 75 mM 3-AT, ... The bait vectors pGBKT7-p53 and pGBKT7-LaminC were co-transformed into AH109 with the prey vector pGADT7-LargeT as positive and negative … http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf biophytis nasdaq direct https://lillicreazioni.com

Functional Characterization of Populus PsnSHN2 in Coordinated …

Webb4 okt. 2013 · The interactions of pHIS2-p53 (three tandem copies of the cis-acting DNA consensus sequence in pHIS2, which is recognized by p53) with the tested TFs were used as negative controls. Two MYC ( ThMYC4 and ThMYC6 GenBank number: JN166788 and JN166790) were identified to bind to the E-box motif, of which ThMYC6 that bound with … Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast cells were dropped onto a selective medium containing synthetic dextrose (SD) without leucine, tryptophan, and histidine but with 10–50 mM 3-aminotriazole (SD/-Leu/-Trp/-His … WebbThe corresponding transformation with pGADT7 prey vector containing WRKY18, WRKY40, or WRKY60 and pHIS2 bait vector containing p53 promoter fragment were used as negative controls. biophytis forum boursorama

The PcbZIP44 transcription factor inhibits patchoulol synthase …

Category:The PcbZIP44 transcription factor inhibits patchoulol synthase …

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Phis2-p53

The bZIP protein from Tamarix hispida, ThbZIP1, is ACGT …

WebbOur Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA interactions. All Matchmaker Gold Systems use Aureobasidin A resistance (AbA r) as a stringent, highly selective, and easy-to-use reporter. Webb27 apr. 2024 · The glycogen synthase 2 (Gys2) and p53 generates a novel negative feedback circuit in which p53 represses Gys2 expression whereas Gys2 can stabilize …

Phis2-p53

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Webb2.制备感受态并将pHis2-DNA转化进入感受态细胞； 3.SD/-Trp平板检测转化结果。 二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板，3-AT的浓度一般设定在50-100mM … WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: …

Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast … WebbFRFS-0353-2 For Epcos saw filter P/N B4959 REV 1.00 Electro-Photonics LLC reserves the right to modify drawings, configurations, specifications, and design at any time without …

Webb12 apr. 2024 · PHIS2-p53+pGAD53m SD-TL SD-TLH 阳性对照 3AT是酵母HIS3蛋白合成的竞争性抑制剂，用于抑制His3基因的泄漏表达。 实验结果如下： 图1：背景筛选结果 结果 … WebbPGADT7-p53 binding to pHIS2-p53 (AD-Rec2-p53+ pHIS2-p53) used as the positive control, AD-Rec2-ScAPD1-like and pHIS2-p53 were used as the negative control. Source publication

Webb9 apr. 2024 · 目的：发现与A相互作用的motif 方法：以构建到pGADT7载体上的A基因为诱饵，筛选酵母单杂交motif文库，经过对阳性克隆的基因检测、DNA测序和比对分析，确定与pGADT7-A相互作用的motif。 材料： 1．诱饵克隆：pGADT7-A 2．诱饵载体：pGADT7 3．文库酵母质粒：motif 4．Clontech酵母单杂交系统 5．培养基

Webb1 mars 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PtrHAT22 were used as the positive and negative control, respectively. 10 −1, 10 −2, and 10 −3 represent solution dilution ratio of transformed Y187 yeast cells. 4. Discussion. dairies queens around u of m twin citieshttp://www.coolaber.com/usr/2160/File/F/F4671.pdf biophyto genesisWebb18 juli 2006 · We generated yeast one-hybrid reporter plasmid by cloning the 356-bp p53 promoter fragment into the pHIS2 reporter plasmid (pHIS2-p53 prom.) and then cotransformed the yeast strain Y187 cells with pHIS2-p53-prom. reporter plasmid and pGAD-Rec2-p53 encoding p53-GAL4 AD fusion. We used pHIS2-p53 cons. reporter … dairies v health and safety executive 2002http://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html dairies of texasWebb14 aug. 2015 · zt028 酵母单杂交载体系统 pHis2 Clontech ... zt1220 pGBK-p53 . zt1221 pGC-silencer-U6-GFP . zt1222 pGIPZ . zt1223 pGL3-FOXO-reporter-luci . zt1224 pGL4.13 . zt1225 pGL4.18 . zt1226 pGL4.19 . zt1227 pGL4.74 . zt1228 pGL5luci . zt1229 ... biophytis share priceWebbp53-His,pLacZ,pHis，pHisi-1. 1、长期保存的储存菌株以冷冻干燥保藏为主，特殊要求的菌种可用其它方法进行保存，保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主，在0℃以下保存，保存期为3－6个月。. 3、应用菌株 … dai riki 285 hook comparison chartWebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: … dairne bound