WebImportantly, CTCF depletion in mESCs did not block cells in a specific phase of the cell cycle and did not induce DNA damage or aneuploidy (Figures S1D and S1E). Cell death … WebSanidas et al. show that, rather than exclusively targeting E2F-promoters, RB associates with specific groups of promoters, enhancers, and insulators to target different sets of genes. Cell-cycle progression redistributes RB …

CTCF functions as a critical regulator of cell-cycle arrest and death ...

WebCTCF loops were shown to be highly dynamic, based on the very transient and cell cycle-dependent binding time of the protein, as measured by live-cell single-molecule … WebSep 15, 2024 · This property is cell cycle dependent; active RB is generated when cells accumulate in G1, during senescence, or during checkpoint-mediated cell-cycle arrest. … raymond e basham

Elevated enhancer-oncogene contacts and higher …

WebJul 9, 2024 · This work reveals that CTCF sites, a key architectural cis-element of the genome, display cell cycle stage-dependent dynamics in factor binding and nucleosome … WebApr 10, 2024 · CTCF, which regulates chromatin architecture, was first identified as an insulator protein that prevents gene expression by blocking enhancer-promoter interactions, and subsequent studies revealed the ability of CTCF to facilitate the formation of TADs and intra-TAD loops. 20, 21 CTCF expression is vital for cell survival, and CTCF-dependent ... WebSeventeen out of 31 patients and both cell lines contained CTCF deletions or mutations, ... Individual samples were barcoded and amplified in a 19-cycle PCR amplification step. Pooled libraries were cleaned up using AMPure XP beads (Beckman Coulter) to enrich for ≥277bp fragments. Sequencing was performed on the Illumina NextSeq500 Desktop ... simplicity simfileshare