Cisplatin cck8
WebMay 28, 2024 · Non-Small Cell Lung Cancer (NSCLC) patients harboring EGFR activating mutations, such as in-frame deletions in exon 19 (Ex19del) or missense mutation in exon … WebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was …
Cisplatin cck8
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WebMar 27, 2024 · Cisplatin-induced cell killing was analyzed by CCK8 assay. Intracellular ROS levels were detected by fluorescence-based flow cytometry analysis. In vitro overexpression and knockdown of GPX1 expression were performed using GPX1 expression vector and siRNA approaches. WebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. Transfection of wild-type p53 gene enhanced the ...
WebCisplatin-induced acute kidney injury in mice was established by intraperitoneal injection of cisplatin. The levels of urea nitrogen and creatinine in serum were measured， renal pathology change was assayed by HE staining， qRT-PCR and Western blot were used to detect the expression levels of PIM1 mRNA and PIM1 protein.
WebCCK8 assay was performed to assess cell proliferation and chemosensitivity of cisplatin-resistant A549/DDP and H1299/DDP cells. Colony-forming assay was utilized to detect colony numbers. Cell migration and invasion ability were measured by transwell assay. The protein levels of LRP, Pgp, MRP1, and SIX1 were examined by Western blot assay. WebObjective: To investigate the regulatory effect of miR-29b on gastric cells' resistance to cisplatin. Methods: The expression of miR-29b in gastric cancer cell line treated with cisplatin concentration gradient was detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. CCK8 was used to …
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WebNational Center for Biotechnology Information chronic fatigue syndrome and pregnancyWebMay 27, 2024 · Uncovering the role of p62 in the development of cisplatin resistance in ovarian cancer. ... (IHC) and its proliferation and invasion effects via CCK8 and transwell assays. Additionally, expression of cyclin D1, MMP2, p‐ERK, and EMT‐related proteins was measured by western blotting. We found that SIX1 had significantly higher expression in ... chronic fatigue syndrome and teensWebCisplatin (CP) is a broad-spectrum antineoplastic agent, used to treat many different types of malignancies due to its high efficacy and low cost. However, its use is largely limited by acute... chronic fatigue syndrome curesWebFeb 28, 2024 · CCK8 was used in ( a) A549 cells and ( b) H460 cells with different concentrations of cisplatin. ( c) CCK8 was used in A549 and H460 cells transfected with miR-494 or controlled with... chronic fatigue syndrome disabilityWebCCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of DNA repair-related proteins. Transwell assay was used to detect cell invasion. Results DNA repair was positively associated with the … chronic fatigue syndrome doctors in michiganWebSep 9, 2016 · The CCK8 assays revealed that, compared to scramble vector, PD-L1 depletion (Fig 2C, left panel) induced more cell growth arrest in the presence of cisplatin (Fig 2C, right panel), suggesting that PD-L1 abrogation restored the sensitivity of H69R and H82R to cisplatin treatment. Thus, PD-L1 upregulation might be required for H69R and … chronic fatigue syndrome dayWeb瑞禧生物提供科研级cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。cisplatin-PEG-NPC顺铂-聚乙二醇-硝基苯碳酸盐试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购cisplatin-PEG-NPC顺铂-聚乙二醇-硝基 ... chronic fatigue syndrome clinic sheffield